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Acidic polysaccharides from Psidium cattleianum (Araçá) BABT
Vriesmann,Lúcia Cristina; Petkowicz,Carmen Lúcia de Oliveira; Carneiro,Paulo Irajara Borba; Costa,Maria Eugênia; Beleski-Carneiro,Eliana.
The aim of this work was to study the acidic polysaccharides of Psidium cattleianum. Pectic fractions were obtained by successive extractions with water, EDTA and sodium carbonate (4 and 25ºC). Monosaccharide composition, total carbohydrates, uronic acids and protein contents were determined for each fraction. The yield of water and EDTA-soluble fractions and high content of uronic acid were consistent with the presence of pectins probably arising from the middle lamellae. On the other hand, sodium carbonate-soluble pectins had a higher neutral sugar content, indicating highly branched polysaccharides in these fractions, consistent with pectins from cell wall.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectins; Acidic polysaccharides; Psidium cattleianum; Cell wall.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200001
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Action of a pancreatin and an Aspergillus oryzae protease on whey proteins: correlation among the methods of analysis of the enzymatic hydrolysates BABT
Morais,Harriman Aley; Silvestre,Marialice Pinto Coelho; Silveira,Josianne Nicácio; Silva,Ana Cristina Simões e; Silva,Viviane Dias Medeiros; Silva,Mauro Ramalho.
The objectives of this study were to hydrolyze whey proteins using a pancreatin and an Aspergillus oryzae protease; to evaluate the degree of hydrolysis (DH) and the peptide profile; and to establish the correlations among the analytical methods. Ten hydrolysates were prepared at different reaction times and the highest DH was obtained by the protein content method. Good correlations (r > 0.87) between the methods of formaldehyde and orthophthalaldehyde (OPA), formaldehyde and osmometry as well as osmometry and OPA were observed using pancreatin. Similar results were obtained between OPA and soluble protein content for the A. oryzae protease. The action of pancreatin produced the highest contents of di- and tripeptides (9.07, 7.12 and 6.46%) and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Whey; Protein Hydrolysates; Degree of Hydrolysis; Peptides; Pancreatin; Aspergillus oryzae Protease.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600014
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Action of Essential Oils on Sprouting of Non-Dormant Potato Tubers BABT
Finger,Fernando Luiz; Santos,Mirelle Mayana de Sousa; Araujo,Fernanda Ferreira; Lima,Paula Cristina Carvalho; Costa,Lucas Cavalcante da; França,Christiane de Fatima Martins; Queiroz,Matheus da Costa.
ABSTRACT The breakdown of dormancy and early sprouting is one of the main causes of losses of stored potatoes. Sprouting control becomes important especially when tubers are submitted to long-term storage for further processing by the potato industry. The objective of this work is to evaluate the effect of organic sprout inhibitors, eugenol and menthol essential oils, on the suppression of growth on non-dormant ‘Asterix’ tubers stored at 8 °C. Administration of eugenol and menthol essential oils reduced the number and the length of sprouts during cold storage up to fifty days. Nevertheless, the essential oils stimulated the break of bud apical dominance, stimulating auxiliary buds growth. The effect of the oils was restricted to inducing necrosis of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum tuberosum; Eugenol; Menthol; Buds; Periderm.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100206
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Action of the extracts of Pluchea sagittalis on the absorptive characteristics of the gastrointestinal tract BABT
Burger,Marilise E.; Baldisserotto,Bernardo; Teixeira,Elisângela P.; Soares,Joceana.
Pluchea sagittalis (Lam.) Cabrera, (quitoco or erva lucera) is commonly used to treat digestive disorders in Southern Brazil and countries of the South Cone. The crude aqueous extracts from the leaves, stalks or flowers were used in acute oral toxicity in mice and in pharmacological studies to determine the gastrointestinal transport of water, sodium, and potassium in rats. The oral administration of 5000 mg/kg of extracts examined did not produce signs of intoxication nor induce the death of any mice during the period of 14 days. The extracts from the leaves and stalk have reduced the absorption of water in the jejunum and jejunum and ileum, respectively with relation to the control. There was an absorption of sodium with the administration of these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pluchea; Ion transport; Gastrointestinal tract absorption; Acute toxicity.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100012
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Actions of crude hydroalcoholic extract of Pfaffia sp on gastrointestinal tract BABT
Freitas,Cristina Setim; Paula,Maria Fernanda Rodrigues de; Rieck,Lia; Marques,Maria Consuelo Andrade.
The plants that compound the Pfaffia genus are used in folk medicine to treat gastric disturbances. This study examined the effects of a crude hydroalcoholic extract of Pfaffia sp on the gastrointestinal tract. Female Wistar rats were pretreated orally (p.o.) with the hydroalcoholic extract of Pfaffia (0.5, 1 and 2 g.kg-1) before the induction of ulcer with hypothermic restraint stress (HRS), ethanol (ET) or indomethacin (IND). Control animals received water (C) or ranitidine (60mg/kg) p.o. The hydroalcoholic extract of Pfaffia (0.5, 1 and 2 mg.kg-1) protected rats against HRS and ET - induced ulcers, but was not able to protect the gastric mucosa against IND - induced ulcers. When injected into the duodenal lumen, the hydroalcoholic extract of Pfaffia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pfaffia; Amaranthaceae; Ulcers; Acid secretion.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300007
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Activities, procedures and doses in pediatric patients due to radiopharmaceuticals BABT
Oliveira,Silvia Maria Velasques de; Boasquevisque,Edson Mendes; Santos,Felipe Sima dos; Biancardi,Rodrigo.
An investigation performed between 2003 and 2005 in sixteen selected public and private institutions in Northeast, Southeast and South geographical regions of Brazil evaluated average organ doses and effective doses in 2,411 pediatric patients due to diagnostic procedures with radiopharmaceuticals. For 1 year, effective doses were greater than literature. For 5 years, differences were noticed between present work and literature for bone scintigraphy, thyroid scintigraphy and 67Ga citrate scintigraphy. These differences may be attributed to the uncertainties in internal dose calculations. High absorved doses in bone surfaces of children due to 67Ga citrate and bone scintigraphy should be evaluated accordingly. Current protocols used recommend standardized...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pediatrics; Oncology; Dosimetry; Radiopharmaceuticals; Nuclear medicine; Radiation protection.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700019
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Activity determination of Na+ K+ - ATPase and Mg++ - ATPase enzymes in the gill of Poecilia vivipara (Osteichthyes, Cyprinodontiformes) in different salinities BABT
Amaral,Marcelo da Cunha; Bonecker,Ana Cristina Teixeira; Ortiz,Cláudio H. D..
This work aimed to know the tolerance mechanisms through the salinity variation by Na+ K+ - ATPase and Mg++ - ATPase and enzymes encountered in the gills of Poecilia vivipara. In field, the presence of this species was observed in salinities of 0 and 28‰. In laboratory, these fish were maintained in aquarium with mean salinity of 30‰ and positive responses were obtained. Some adult specimens, collected in a lagoon of the Coqueiros Beach, were utilized as matrixes. In the experiments the specimens used were those born in the test aquarium. For each salinity studied three replicates were made with three specimens for each one. The alevins were maintained in salinities of 5, 10, 15, 20, 25, 30 and 35‰ during a month for adaptation. Gills were extracted in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Poecilia vivipara; Na+ K+ ATPase; Mg++ ATPase; Osmoregulation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100001
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Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheres BABT
Chen,Meng-Yan; Zheng,Wei; Dong,Qiu-Yue; Li,Zhen-Hua; Shi,Lu-E; Tang,Zhen-Xing.
In this work, alginate-whey protein was used as wall materials for encapsulating Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus). The characteristics of encapsulated and free L. bulgaricus showed that the free L. bulgaricus lost viability after 1 min exposure to simulated gastric fluid (SGF) at pH 2.0 and 2.5. However, the viability of encapsulated L. bulgaricus did not decrease in SGF at pH 2.5 for 2 h incubation. The viable numbers of encapsulated L. bulgaricus decreased less than 1.0 log unit for 2 h incubation in SGF at pH 2.0. For bile stability, only 1.2 log units and 2.0 log units viability of the encapsulated L. bulgaricus was lost in 1 and 2% bile for 1 h exposure, respectively, compared with no survival of free L. bulgaricus under the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Encapsulation; Alginate; Whey protein; Probiotics; Stability.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500736
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Activity of enzymes associated with the enzymatic browning of minimally processed potatoes BABT
Vitti,Maria Carolina Dario; Sasaki,Fabiana Fumi; Miguel,Patrícia; Kluge,Ricardo Alfredo; Moretti,Celso Luiz.
The purpose of the present study was to evaluate the effect of different potato cultivars and storage temperatures on the specific activity of phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) in minimally processed potatoes. Potato cultivars Agata, Asterix and Monalisa were selected, washed, peeled, diced, sanitized, centrifuged, vacuum- packed and stored at 5 and 15°C for 9 and 5 days, respectively. There was an increase in the enzymatic activity in all the cultivars stored at 15°C. The cultivars 'Agata' and 'Asterix' stored at 5ºC did not differ significantly between them for the PAL, PPO and POD activities. The PAL, PPO and POD activities were also influenced by the storage temperature. The cultivars Agata and Asterix...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum tuberosum L; Phenylalanine ammonia-lyase; Polyphenol oxidase; Peroxidase; Minimal processing.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500016
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Açúcares neutros, ácidos urônicos e condutividade elétrica do exsudato de sementes de algodoeiro (gossypium hirsutum l.), em função do tempo de duração do deslintamento químico BABT
Chitarra,Luiz Gonzaga; Chitarra,Adimilson Bosco; Machado,José da Cruz; Menezes,Josivan Barbosa.
The objective of this work was to evaluate the efficiency of delinting time of cottonseeds through the electrical conductivity and the quantity of neutral sugars and uronic acids leaked from the seeds during imbibition in water, in function of the fractioning in water and the artificial ageing. The seeds were delinted with commercial sulphuric acid for 1.5 and 4.5 minutes, separeted according to the sedimentation fractions in water and submitted to artificial ageing for 0, 72 and 96 hours. Electrical conductivity, contents of neutral sugars and uronic acids were determinated from the exudate resulting from the seeds imbibition in relation different treatments. The results showed that the delinting time for 1.5 minutes provided higher values of electrical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Açúcares neutros; Ácidos urônico; Semente de algodão deslintada; Condutividade elétrica.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100013
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Acute intraperitoneal mercury chloride contamination and distribution in liver, muscle and gill of a neotropical fish Hoplias malabaricus (BLOCK, 1794) BABT
Jesus,Taise Bomfim de; Almeida,Priscila Gontijo Aguiar de; Vergílio,Cristiane dos Santos; Machado,André Luiz dos Santos; Carvalho,Carlos Eduardo Veiga de.
The present study investigated with the distribution of mercury chloride in muscle, liver and gills of Hoplias malabaricus contaminated through intraperitoneal injection (6 µg in 0.1mL of PBS) for a period of 24, 48, 72 and 96h. The liver, gill and muscle were analyzed for mercury content in an ICP/AES (Varian Liberty II) with vapor generating accessory (VGA 77). The muscle and liver tissues presented the same contamination pattern increasing concentrations in 24 h of exposure with a decrease in Hg concentration with 72 h and a new increase in Hg concentrations with 96 h of exposure. The Hg concentrations in contaminated organisms were always higher than the control although only for liver samples the difference was statistically significant. Liver samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inorganic mercury; Liver; Acute effects; Intraperitoneal contamination.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200021
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Adiabatic differential scanning calorimetric study of divalent cation induced DNA - DPPC liposome formulation compacted for gene delivery BABT
Süleymanoglu,Erhan.
Complexes between nucleic acids and phospholipid vesicles have been developed as stable non-viral gene delivery vehicles. Currently employed approach uses positively charged lipid species and a helper zwitterionic lipid, the latter being applied for the stabilization of the whole complex. However, besides problematic steps during their preparation, cationic lipids are toxic for cells. The present work describes some energetic issues pertinent to preparation and use of neutral lipid-DNA self-assemblies, thus avoiding toxicity of lipoplexes. Differential scanning calorimetry data showed stabilization of polynucleotide helix upon its interaction with liposomes in the presence of divalent metal cations. It is thus possible to suggest this self-assembly as an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nucleic acid-phospholipid interactions; Divalent cation-induced complex formation; Phase transitions; Microcalorimetry.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600007
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Administration of venlafaxine after chronic methadone detoxification blocks post-depression relapse in rats BABT
Fadaei-Kenarsary,Meysam; Moghaddam,Hadi Fathi; Mansouri,Seyyed Mohammad Taghi; Farbood,Yaghoob.
ABSTRACT Relapse is highly prevalent after detoxification and depression. Due to the advantages of venlafaxine compared with other antidepressants, it is expected that venlafaxine administration may reduce relapse after detoxification and depression. This study aimed to evaluate the effects of venlafaxine on depression-induced relapse to morphine dependence after methadone detoxification. Eighty Sprague-Dawley rats were habituated and conditioned with morphine (10 mg/kg, S.C., for 4 days). After that, primary forced swimming and conditioned place preference (CPP) were tested. They were followed by methadone (70 mg/kg/day, P.O., for 7 days) administration, extinguishing, forced swimming stress (FSS) and administration of venlafaxine (80 mg/kg/day, I.P., for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphine; Methadone; Depression; Relapse; Venlafaxine; Rat.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100311
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Adsorption, immobilization and activity of cellulase in soil: the impacts of maize straw and its humification BABT
Sinegani,Ali Akbar Safari; Sinegani,Mahboobe Safari.
The present work aimed to study some aspects of sorption and immobilization of cellulase molecules on soil components by the analysis of the reactions of cellulase in a soil treated with different levels of maize residue and incubated for 90 days. The analysis of variance showed that the effects of the treatments of maize straw, incubation time and their interaction on cellulase adsorption, desorption and immobilization were statistically significant. The adsorption and immobilization capacities of soil by application of maize straw increased significantly. However they decreased with decreasing the soil organic matter (SOM) after 45 days of incubation. The desorption of adsorbed cellulase molecules from the soil by washing with distilled water depended on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maize straw; Humification; Cellulase; Sorption; Immobilization; Activity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600001
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Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin BABT
Manera,Ana Paula; Kamimura,Eliana Setsuko; Brites,Luciana Machado; Kalil,Susana Juliano.
Amyloglucosidase enzyme was produced by Aspergillus niger NRRL 3122 from solid-state fermentation, using deffated rice bran as substrate. The effects of process parameters (pH, temperature) in the equilibrium partition coefficient for the system amyloglucosidase - resin DEAE-cellulose were investigated, aiming at obtaining the optimum conditions for a subsequent purification process. The highest partition coefficients were obtained using 0.025M Tris-HCl buffer, pH 8.0 and 25ºC. The conditions that supplied the highest partition coefficient were specified, the isotherm that better described the amyloglucosidase process of adsorption obtained. It was observed that the adsorption could be well described by Langmuir equation and the values of Qm and Kd...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amyloglucosidase; Adsorption isotherm; Kinetics adsorption; Ion exchange resin; Purification.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500019
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Adsorption of glyphosate on clays and soils from Paraná State: effect of pH and competitive adsorption of phosphate BABT
Cruz,Lútecia Hiera da; Santana,Henrique de; Zaia,Cássia Thaïs Bussamra Vieira; Zaia,Dimas Augusto Morozin.
This work showed that the adsorption of glyphosate (GPS) depends on surface area for clays and amount of clays and CEC for soils. Organic matter (OM) had a secondary role in the adsorption of GPS on soils. The adsorption of GPS on soils from Londrina and Floraí counties and clays (montmorillonite, kaolinite) decreased when pH increased, however, for bentonite clay and soil from Tibagi county was kept constant. For the soils, the competitive adsorption between GPS and phosphate showed that displace of GPS by phosphate was related to the amount of clays, CEC and pH. GPS was not easily displaced by phosphate on the clays. The FT-IR spectra of the soils and clays showed that soil from Londrina resembled kaolinite. Thus, this could explain the results of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glyphosate; Adsorption; Clays; Soils.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300004
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Adsorption of natural dyes on clay fixed on polymers BABT
Lopes,Toni Jefferson; Gonçalves,Odinei Hess; Barros,Ricardo Barbosa; Capelli,Francine Carla Rodrigues; Machado,Ricardo Antonio Francisco; Quadri,Mara Gabriela Novy; Quadri,Marintho Bastos.
The main purpose of this work was to produce a pellet to minimize undesirable effects in fixed beds, such as colmatation, through clay fixation on the surface of polymeric particles. Preliminary experiments were carried out by heating the clay, namely Tonsil Terrana 580FF, in order to observe variations on its capacity to adsorb anthocyanins derived from red cabbage. Clays was fixed on five samples of high density polyethylene (HDPE) at 180°C -210°C for two hours. The morphological analyses of the resulting particles were accomplished through Scanning Electron Microscopy (SEM). The experiments demonstrated an increase on clay's adsorptive capacity of 15.65% at 120°C for 30 hours and 16.80% at 170°C for two hours. The SEM analysis showed that the clay...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adsorption; Clay; Polymer; Anthocyanin/red cabbage.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400034
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Adult feeding and mating effects on the biological potential and parasitism of Trichogramma pretiosum and T. acacioi (Hym.: Trichogrammatidae) BABT
Pratissoli,Dirceu; Oliveira,Harley Nonato de; Polanczyk,Ricardo Antonio; Holtz,Anderson Mathias; Bueno,Regiane Cristina Oliveira de Freitas; Bueno,Adeney de Freitas; Gonçalvez,José Roberto.
This work was carried out to study the effects of adult feeding and mating on the biological potential and parasitism of Trichogramma pretiosum and T. acacioi (Hymenoptera: Trichogrammatidae) to improve their use in biological control programs. Both species presented higher parasitism and longevity whenever adults were fed. Fed and unmated T. pretiosum females led to low parasitism whereas T. acacioi females did not present parasitism whatsoever. Egg viability of T. pretiosum was similar for fed and mated individuals, but T acacioi showed lower values for this parameter when unfed and without mating. Unmated females produced only males while mated ones had more than 60% female descendents for both Trichogramma species. Therefore, mated and fed female...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Parasitoid feeding; Parasitoid mating.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500001
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Advances in bacterial specific imaging BABT
Wareham,David; Michael,J.; Das,Satya.
Nuclear medicine is a powerful diagnostic technique able to detect inflammatory foci in human disease. A wide range of agents have been evaluated for their ability to distinguish lesions due to microbial infection from those due to sterile inflammation. Advances continue to be made on the use of radiolabelled antibiotics which as well as being highly specific in the diagnosis of infection may be useful in monitoring the treatment and course of disease. Here we provide an update on in-vitro and clinical studies with a number of established and novel radiopharmaceuticals
Tipo: Info:eu-repo/semantics/article Palavras-chave: Radiopharmaceuticals; Infection specific imaging; Radiolabelled anti-infectives; 99mTc- ciprofloxacin.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700021
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Advantages and Applications of Cryopreservation in Fisheries Science BABT
Tsai,S.; Lin,C..
Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high chilling sensitivity and low membrane permeability. However, cryopreservation of isolated embryonic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryopreservation; Sperm; Embryo; Oocyte; Blastomere.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300014
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